Membranas de celulose bacteriana e nanopartículas de prata para aplicações em tratamento de feridas e queimaduras
DOI:
https://doi.org/10.36557/2674-8169.2024v6n8p3595-3612Keywords:
Cicatrização, Curativos, terapia antimicrobianaAbstract
Introduction: Burns are considered a significant medical and social challenge in Brazil and worldwide, involving a high incidence, morbidity and mortality rates. One of tha main complications is related to the secondary infection of these injuries. Therefore dressings with antimicrobial action can be valuable asset in treating this condition, promoting healing. The introduction of topical silver in burn treatment was a milestone in reducing morbidity and mortality. Bacterial celulose membranes are notable in the Heath field for their biocompatibility and easy use incorporating componentes. This study developed bacterial celulose membranes impregnated with silver nanoparticles and tested their antimicrobial action against the main pathogens colonizing wounds and burns. Methods: Bacterial celulose membranes were produced in a synthetic culture. The membranes underwent various processes, including washing, purification, decellularization, drying at room temperature, and impregnation with silver nanoparticles. Sensitivity testing of the membranes was conducted against Staphylococcus aureus ATCC 23925 (Gram-positive) and Pseudomonas aeruginosa ATCC (Gram-negative), common bacterias in secondary infections of burns, using Chloramphenicol as a control. Results: The test evaluated the mean inhibition halo (MIH) in millimeters (mm). Against S. aureus, the MIH of the control was 17.2 ± 3,45mm, while the membranes MIH was 7,25 ± 0,1mm. Against P. aeruginosa, the control obtained a MIH of 32,88 ± 0,91mm; and the bacterial celulose membranes had a MIH of 8,91 ± 0,69mm. Conclusion: the developed membranes exhibit antimicrobial activity against S. aureus of 42.12% and 27% against P. aeruginosa compared to the control (Cloranfenicol) demonstrating a significant inhibitory action against the analyzed pathogens. For their future practical application, further studies about their properties should be conduced.
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Copyright (c) 2024 Letícia Garcia Rabelo, Danilo José Machado de Abreu, Carlos Henrique de Oliveira Magno, Laryssa Chaves Vieira , Roberta Hilsdorf Piccoli, Juliano Elvis de Oliveira
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