Cryopreservation in Dentistry: Cryobiological Paradigms, Current Applications, and Transformative Prospects at the Forefront of Dental Regenerative Therapeutics
DOI:
https://doi.org/10.36557/2674-8169.2025v7n7p1573-1587Palavras-chave:
Cryopreservation, Regenerative Dentistry, Odontogenic Stem Cells, Dental Biobanks, Applied Cryobiology, Orofacial Tissue EngineeringResumo
Objective: This study aimed to review the scientific literature on cryopreservation applied to dentistry, with a special focus on the preservation of dentoalveolar structures and mesenchymal stem cells of dental origin. The aim was to understand the biological foundations involved, technological advances, and future prospects for the creation of autogenous tooth banks and bioengineered constructs. Methodology: A systematic search was conducted in PubMed, Scopus, and Web of Science, considering publications published between 2005 and 2025. The descriptors used were: cryopreservation, dental stem cells, tooth bank, and tissue engineering. Original articles, systematic reviews, and experimental trials related to the cryopreservation of dental stem cells, whole teeth, and dentoalveolar tissues were included. The data were organized into categories: principles of cryobiology, technical protocols, current clinical applications, and potential advances. Results: The reviewed literature confirms that cryopreservation is already a reality for the conservation of isolated dental stem cells, using protocols that combine cryoprotectants such as DMSO with controlled cooling rates, minimizing damage from ice crystallization. However, the preservation of whole teeth faces significant technical challenges due to tissue heterogeneity and limited diffusion of cryoprotectants. Experimental research indicates promising advances in tooth banks for reimplantation and bioengineered constructs, although they are still far from consolidated clinical application. Conclusion: Cryopreservation is emerging as a transformative strategy in regenerative and personalized dentistry, integrating concepts of cryobiology with advanced tissue engineering techniques. However, clinical consolidation depends on overcoming technical limitations and overcoming bioethical and regulatory barriers. In this context, the concept of "cryodentistry" emerges, pointing to a future in which the preservation of tissues and whole teeth could enhance regenerative therapies, reinforcing the role of cryopreservation as a central tool in future dentistry.
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Duailibi, S. E., Duailibi, M. T., Zhang, W., Asrican, R., Vacanti, J. P., & Yelick, P. C. (2008). Bioengineered Dental Tissues Grown in the Rat Jaw. Journal of Dental Research, 87(8), 745–750. https://doi.org/10.1177/154405910808700811
Gao, D., & Critser, J. K. (2000). Mechanisms of Cryoinjury in Living Cells. ILAR Journal, 41(4), 187–196. https://doi.org/10.1093/ilar.41.4.187
Gronthos, S., Mankani, M., Brahim, J., Robey, P. G., & Shi, S. (2000). Postnatal human dental pulp stem cells (DPSCs) in vitro and invivo. Proceedings of the National Academy of Sciences, 97(25), 13625–13630. https://doi.org/10.1073/pnas.240309797
Hackney, K. J., & English, K. L. (2014). Protein and Essential Amino Acids to Protect Musculoskeletal Health during Spaceflight: Evidence of a Paradox? Life : Open Access Journal, 4(3), 295–317. https://doi.org/10.3390/life4030295
Hilkens, P., Driesen, R. B., Wolfs, E., Pascal Gervois, Vangansewinkel, T., Ratajczak, J., Dillen, Y., Annelies Bronckaers, & Ivo Lambrichts. (2016). Cryopreservation and Banking of Dental Stem Cells. Biobanking and Cryopreservation of Stem Cells, 1(1), 199–235. https://doi.org/10.1007/978-3-319-45457-3_17
Kaku, M., Kamada, H., Kawata, T., H. Koseki, S. Abedini, Kojima, S., M. Motokawa, Fujita, T., J. Ohtani, N. Tsuka, Matsuda, Y., H. Sunagawa, Hernandes, R. A. M., N. Ohwada, & K. Tanne. (2010). Cryopreservation of periodontal ligament cells with magnetic field for tooth banking. Cryobiology, 61(1), 73–78. https://doi.org/10.1016/j.cryobiol.2010.05.003
Mazur, P. (1970). Cryobiology: The Freezing of Biological Systems. Science, 168(3934), 939–949. https://doi.org/10.1126/science.168.3934.939
Miura, M., Gronthos, S., Zhao, M., Lu, B., Fisher, L. W., Robey, P. G., & Shi, S. (2003). SHED: Stem cells from human exfoliated deciduous teeth. Proceedings of the National Academy of Sciences, 100(10), 5807–5812. https://doi.org/10.1073/pnas.0937635100
Morsczeck, C., Götz, W., Schierholz, J., Zeilhofer, F., Kühn, U., Möhl, C., Sippel, C., & Hoffmann, K. H. (2005). Isolation of precursor cells (PCs) from human dental follicle of wisdom teeth. Matrix Biology, 24(2), 155–165. https://doi.org/10.1016/j.matbio.2004.12.004
Nakashima, M., Iohara, K., Murakami, M., Nakamura, H., Sato, Y., Ariji, Y., & Matsushita, K. (2017). Pulp regeneration by transplantation of dental pulp stem cells in pulpitis: a pilot clinical study. Stem Cell Research & Therapy, 8(1). https://doi.org/10.1186/s13287-017-0506-5
Pegg, D. E. (2002). The History and Principles of Cryopreservation. Seminars in Reproductive Medicine, 20(1), 005-014. https://doi.org/10.1055/s-2002-23515
Perry, B. C., Zhou, D., Wu, X., Yang, F., Byers, M., T.-M. Gabriel Chu, J. Jeffrey Hockema, Woods, E. J., & Goebel, W. (2008). Collection, Cryopreservation, and Characterization of Human Dental Pulp–Derived Mesenchymal Stem Cells for Banking and Clinical Use. Tissue Engineering Part C: Methods, 14(2), 149–156. https://doi.org/10.1089/ten.tec.2008.0031
Rai, S., Kaur, M., & Kaur, S. (2013). Applications of stem cells in interdisciplinary dentistry and beyond: An overview. Annals of Medical and Health Sciences Research, 3(2), 245. https://doi.org/10.4103/2141-9248.113670
Rall, W. F., & Fahy, G. M. (1985). Ice-free cryopreservation of mouse embryos at −196 °C by vitrification. Nature, 313(6003), 573–575. https://doi.org/10.1038/313573a0
Seo, B.-M., Miura, M., Gronthos, S., Mark Bartold, P., Batouli, S., Brahim, J., Young, M., Gehron Robey, P., Wang, C. Y., & Shi, S. (2004). Investigation of multipotent postnatal stem cells from human periodontal ligament. The Lancet, 364(9429), 149–155. https://doi.org/10.1016/s0140-6736(04)16627-0
Sonoyama, W., Liu, Y., Fang, D., Yamaza, T., Seo, B.-M., Zhang, C., Liu, H., Gronthos, S., Wang, C.-Y., Shi, S., & Wang, S. (2006). Mesenchymal Stem Cell-Mediated Functional Tooth Regeneration in Swine. PLoS ONE, 1(1), e79. https://doi.org/10.1371/journal.pone.0000079
Woods, E. J., Perry, B. C., J. Jeffrey Hockema, Larson, L. A., Zhou, D., & W. Scott Goebel. (2009). Optimized cryopreservation method for human dental pulp-derived stem cells and their tissues of origin for banking and clinical use. Cryobiology, 59(2), 150–157. https://doi.org/10.1016/j.cryobiol.2009.06.005
Young Hak Oh, Zhong, M., Hong, J., Eun Ju Lee, Seung Jong Lee, & Kim, J. (2005). Cryopreservation of human teeth for future organization of a tooth bank—A preliminary study. Cryobiology, 51(3), 322–329. https://doi.org/10.1016/j.cryobiol.2005.08.008
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Copyright (c) 2025 Alessandra Fátima Britto Barbosa Matos, José Cardoso de Holanda Filho, Marvin Gonçalves Duarte, Ailton Coelho de Ataíde Filho, Luciano Barreto Silva
Este trabalho está licenciado sob uma licença Creative Commons Attribution 4.0 International License.
Os autores são detentores dos direitos autorais mediante uma licença CCBY 4.0.
