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Abstract
Hamelia patens Jacq (H. patens), a species of medicinal plant belonging to the Rubiaceae family, is known for its easy growth in different types of soil, its healing properties for many pathologies, from exposed wounds to tumors and diabetes. It is a very versatile plant, used for different purposes, many tests have already been carried out proving its effectiveness in important treatments, using all its parts, such as root, leaves and flower. The objective of this study was to evaluate the in vitro antioxidant capacity and cytotoxic effects of H. patens ethanolic extract on NIH/3T3 cells. The analysis of the plant's phytochemical compounds was carried out using HPLC (High Performance Liquid Chromatography). Cell viability was assessed by the MTT assay. Cell proliferation was performed using the crystal violet method. Antioxidant activity was evaluated using the free radical scavenging method (DPPH). The ultraviolet spectra obtained by HPLC showed characteristics of flavonoids. Cell viability was significantly reduced in cells treated with ethanolic extract of H. patens (EEHP) at concentrations of 100 µg/mL, 500 µg/mL and 1000 µg/mL. Cell proliferation demonstrated a significant increase in cells treated with 10 µg/mL. Our results demonstrate that EEHP has flavonic compounds, low cytotoxicity at concentrations of 10 µg/mL and 50 µg/mL, and a significant proliferative power, which suggests it has healing properties and antioxidant power. Furthermore, our results contribute to the search for new therapeutic targets and future studies related to this species.
References
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Copyright (c) 2023 Gabriela Rodrigues Oliveira, Vinícius Bednarczuk de Oliveira, Regiane Stafim da Cunha, Edson José Mazarotto, Paulo Cézar Gregório, Giane Favretto