Abstract
The aim of this study was to isolate and identify Candida species from the oral cavity of den-ture wearers with denture-related stomatitis who were attended at the University Federalof Pará (Belém City, Pará State, Brazil). A total of 36 denture wearers with denture-relatedstomatitis were included, and type I (50%), type II (33%) and type III (17%) stomatitis wereobserved. Candida spp. were isolated from 89% of the cases and included five differentCandida species. C. albicans was the most frequently recovered species (78% of the cases),followed by C. famata and C. tropicalis. We observed a significant association between Candidaspecies isolation and unsatisfactory denture condition (p = 0.0017). Our results demonstratedthe highly frequency of Candida species isolation in denture wearers with denture-relatedstomatitis and showed the relationship between these species and poor denture maintence.
References
- M.E. Maluche, J.I. Santos. Candida spp e infecções hospitalares: aspectos epidemiológicos e laboratoriais. RBAC, 40 (2008), pp. 65-67
- B.W. Loster, J. Loster, A. Wieczorek, W. Ryniewicz. Mycological analysis of the cavity of patients using acrylic removable dentures. Gastroenterol Res Pract, (2012), p. 951572
- O. Abaci, B. Ozturk, H. Boyacioglu. Determining Candida spp. Incidence in denture wearers. Mycopathologia, 169 (2010), pp. 365-372
- K. Bulad, R.L. Taylor, J. Verran, J.F. McCord. Colonization and penetration of denture soft lining materials by Candida albicans. Dent Mater, 20 (2004), pp. 167-175
- S. Jeganathan, C.C. Lin. Denture stomatitis – a review of the etiology, diagnosis and management. Aust Dent J, 37 (1992), pp. 107-114
- B.C. Webb, C.J. Thomas, M.D.P. Willcox. Candida-associated denture stomatitis. Aetiology and management: a review. Part I. Factors influencing distribution of Candida species in the oral cavity. Aust Dent J, 43 (1998), pp. 45-50
- S. Silva, M. Henriques, A. Hayes, R. Oliveira, J. Azeredo, D.W. Williams. Candida glabrata and Candida albicans co-infection of an in vitro oral epithelium. J Oral Pathol Med, 40 (2011), pp. 421-427
- M.G. Waters, D.W. Williams, R.G. Jagger, M.A. Lewis. Adherence of Candida albicans to experimental denture soft lining materials. J Prosthet Dent, 77 (1997), pp. 306-312
- L. Gendreau, Z.G. Loewy. Epidemiology and etiology of denture stomatitis. J Prosthodont, 20 (2011), pp. 251-260
- L.C. Spolidorio, V.R.G. Martins, R.D. Nogueira, D.M.P. Spolidorio. Frequência de Candida spp em biópsias de lesões da mucosa oral. Pesq Odont Bras, 17 (2003), pp. 89-93
- E.A.F.A. Navas, A.C. Inocêncio, J. Dias, et al. Oral distribution of Candida species and presence of oral lesions in Brazilian leprosy patients under multidrug therapy. J Oral Pathol Med, 38 (2009), pp. 764-767
- P.A. Reichart, L.P. Samaranayake, C.H. Bendick, A.M. Schmidt-Westhausen, J.A. Jayatilake. Prevalence of oral Candida species in leprosy patients from Cambodia and Thailand. J Oral Pathol Med, 36 (2007), pp. 342-346
- M. Martins, M. Henriques, A.P. Ribeiro, et al .Oral Candida carriage of patients attending a dental clinic in Braga, Portugal. Rev Iberoam Micol, 27 (2010), pp. 119-124
- D.R. Snydman. Shifting patterns in the epidemiology of nosocomial Candida infections. Chest, 123 (2003), pp. 500S-503S
- P.V. Sanitá, A.C. Pavarina, E.T. Giampaolo, et al. Candida spp. prevalence in well controlled type 2 diabetic patients with denture stomatitis. Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 111 (2011), pp. 726-733
- A.V. Newton. Denture sore mouth: a possible etiology. Br Dent J, 112 (1962), pp. 357-360
- J. Sambrook, E.F. Fritsch, T. Maniatis. Molecular Cloning: A Laboratory Manual
(2nd ed.), Cold Spring Habor Laboratory Press (1986)
- B.M. Mannarelli, C.P. Kurtzman. Rapid identification of Candida albicans and other human pathogenic yeasts by using short oligonucleotides in a PCR. J Clin Microbiol, 36 (1998), pp. 1634-1641
- G. Luo, T.G. Mitchell. Rapid identification of pathogenic fungi directly from cultures by using multiplex PCR. J Clin Microbiol, 40 (2002), pp. 2860-2865
- K. Zomorodian, N.N. Haghighi, N. Rajaee, et al. Assessment of Candida species colonization and denture-related stomatitis in complete denture wearers. Med Mycol, 49 (2011), pp. 208-211
- R.C. Ferreira, C.S. Magalhães, N.A. Moreira. Oral mucosal alterations among the institutionalized elderly in Brazil. Braz Oral Res, 24 (2010), pp. 296-302
- T. Pereira-Cenci, A.A. Cury, M.S. Cenci, R.C. Rodrigues-Garcia. In vitro Candida colonization on acrylic resin and denture liners: influence of surface free energy, roughness, saliva, and adhering bactéria. Int J Prosthodont, 20 (2007), pp. 308-310
- S.E. Park, R. Blissett, S.M. Susarla, H.-P. WeberCandida albicans adherence to surface-modified denture resin surfaces. J Prosthodont, 17 (2008), pp. 365-369
- A.M.G. Darwazeh, M.M. Hammad, A.A. Al-Jamaei. The relationship between oral hygiene and oral colonization with Candida species in healthy adult subject. Int J Dent Hyg, 8 (2010), pp. 128-133.
- S. Dagistan, A.E. Aktas, F. Caglayan, A. Ayyildiz, M. Bilge. Differential diagnosis of denture-induced stomatitis, Candida and their variations in patients using complete denture: a clinical and mycological study. Mycoses, 52 (2008), pp. 266-271
- P.Y. Ge, X.G. He, T. Lin, X.G. Lu, N.Y. Shen, W.D. LiuFirst isolation of Candida dubliniensis from oral cavities of dermatological patients in Nanjing, China. Mycopathologia, 172 (2011), pp. 465-471
- T. Kadir, R. Pisiriciler, S. Akyüz, A. Yarat, N. Emekli, A. Ipbüker. Mycological and cytological examination of oral candida carriage in diabetic patients and non-diabetic control subject: thorough analysis of local etiologic and systemic factors. J Oral Rehabil, 29 (2002), pp. 452-457
- J.P. Lyon, L.M. Moreira, M.A.G. Cardoso, J. Saade, M.A. Resende. Antifungal susceptibility profile of Candida spp. oral isolates obtained from denture wearers. Braz J Microbiol, 39 (2008), pp. 668-672
- E.A. Menezes, M.S. Cavalcante, R.B. Farias, et al. Frequency and enzymatic activity of Candida albicans isolates from the buccal mucosa of children of a day-care center of the city hall of Fortaleza, Ceará, Brazil. J Bras Pathol Med Lab, 41 (2005), pp. 9-13
- F.R. Pires, E.B. Santos, P.R. Bonan, O.P. De Almeida, M.A. Lopes. Denture stomatitis and salivary Candida in Brazilian edentulous patients. J Oral Rehabil, 29 (2002), pp. 1115-1119
- P.L. Sant’Ana, E.P. Milan, R. Martine, et al. Multicenter Brazilian Study of oral Candida species isolated from Aids patients. Mem Inst Oswaldo Cruz, 97 (2002), pp. 253-257
- Gauch, Lurdete Maria Rocha, et al. "Isolation of Candida spp. from denture-related stomatitis in Pará, Brazil." brazilian journal of microbiology 49.1 (2018): 148-151.
Este artigo é uma cópia com adaptação para o português do Artigo Original "Gauch, Lurdete Maria Rocha, et al. "Isolation of Candida spp. from denture-related stomatitis in Pará, Brazil." brazilian journal of microbiology 49.1 (2018): 148-151." Esta Cópia com adaptação para o Português segue os preceitos Creative Commons CC BY-NC 4.0 ( https://creativecommons.org/licenses/by/4.0/deed.pt_BR ) disponibilizado pelo periódico responsável pela publicação original ( https://www.sciencedirect.com/science/article/pii/S1517838217307001 ).
CONFLITO DE INTERESSES
Os autores declaram não haver conflitos de interesse
CONFLICT OF INTERESTS
The authors declare no conflicts of interest

This work is licensed under a Creative Commons Attribution 4.0 International License.
Copyright (c) 2020 Lurdete Maria Rocha Gauch, Simone Soares Pedrosa, Fabíola Silveira-Gomes, Renata Antunes Esteves, Silvia Helena Marques da Silva