Keywords
Durapatite
Strontium
How to Cite
Abstract
With the advancement of research in biomaterials, it has been suggested that the best osteoconductivity of hydroxyapatite would be achieved if its crystal were closer to the structure, size and morphology of biological apatite, that is why nano-hydroxyapatite (nano-HA) is of great importance. current interest. Strontium ions are known to reduce bone resorption, induce osteoblastic activity and stimulate bone formation. The aim of this study was to evaluate biocompatibility and osteoconduction in surgical defects filled with nano-hydroxyapatite microspheres containing 1% strontium (nano-SrHA), stoichiometric nano-HA microspheres (nano-HA) compared to the clot (control) . Four Santa Inês sheep, weighing an average of 32 kg, were anesthetized and submitted to three 2 mm diameter perforations in the medial face of the tibia. The surgical defects were filled with blood clot, microspheres of Sr-HA 1% and microspheres of HA. After 30 days the samples were drawn (6 mm), decalcified, processed for inclusion in paraffin and stained with hematoxylin and eosin (HE) for histological evaluation with light microscopy. All groups revealed bone neoformation from the periphery to the center of the defect, with the nano-SrHA group being less intense among those studied. Presence of a discrete mononuclear inflammatory infiltrate in all experimental groups. Giant foreign body cells were only observed in the HA group. Areas of bone neoformation were observed in close contact with both biomaterials. According to the results obtained, microspheres of HA and SrHA 1% are biocompatible and have osteoconductive properties.
References
Augat P, Margevicius K, Simon J, Wolf S, Suger G, Claes L. Local tissue properties in bone healing: influence of size and stability of the osteotomy gap. J Orthop Res. 1998; 16 (4): 475-81.
Blake GM, Zivanovic MA, McEwan AJ, Ackery DM. Sr-89 therapy: strontium kinetics in disseminated carcinoma of the prostate. Eur J Nucl Med. 1986; 12 (9): 447–54.
Canalis E, Hott M, Deloffre P, Tsouderos Y, Marie PJ. The divalent strontium salt S12911 enhances bone cell replication and bone formation in vitro . Bone. 1996; 18 (6): 517–23.
Capuccini C, Torricelli P, Sima F, Boanini E, Ristoscu C, Bracci B, et al. Strontium-substituted hydroxyapatite coatings synthesized by pulsed-laser deposition: in vitro osteoblast and osteoclast response. Acta Biomater. 2008; 4 (6); 1885-93.
Christoffersen J, Christoffersen MR, Kolthoff N, Bärenholdt O. Effects of strontium ions on growth and dissolution of hydroxtapatite and on bone mineral detection. Bone. 1997; 20 (1): 47-54.
Dagang G, Kewei X, Yong H. The influence of Sr doses on the in vitro biocompatibility and in vivo degradability of single-phase Sr-incorporated HAP cement. J Biomed Mater Res A. 2008; 86 (4): 947-58.
Guo D, Xu K, Zhao X, Han Y. Development of a strontium-containing hydroxyapatite bone cement. Biomaterials. 2005; 26 (19): 4073-83.
Hu R, Lin CJ, Shi HY. A novel ordered nano hydroxyapatite coating electrochemically deposited on titanium substrate. J Biomed Mater Res A. 2007; 80 (3): 687-92.
Huang Z, Tian J, Yu B, Xu Y, Feng Q. A bone-like nano-hydroxyapatite / collagen loaded injectable scaffold. Biomed Mater. 2009; 4 (5): 055005.
Lekovic V, Kenney EB, Weinlaender M, Han T, Klokkevold P, Nedic M, et al. A bone regenerative approach to alveolar ridge maintenance following tooth extraction. Report of 10 cases. J Periodontol. 1997; 68 (6): 563-70.
Marie PJ, Ammann P, Boivin G, Rey C. Mechanisms of action and therapeutic potential of strontium in bone. Calcif Tissue Int. 2001; 69 (3): 121-9.
Melloning JT, Triplett RG. Guided tissue regeneration and endosseous dental implants. Int J Periodontics Restorative Dent. 1993; 13 (2): 109-19.
Nunamaker DM. Experimental models of fracture repair. J Clin Orthop Relat Res. 1989; (355 Suppl): 56-65.
Nuss KMR, Auer JA, Boss A, von-Rechenberg B. An animal model in sheep for biocompatibility testing of biomaterials in cancellous bones. BMC Musculoskeletal Disord. 2006; 7: 67.
Patel N, Brooks RA, Clarke MT, Lee PM, Rushton N, Gibson IR, et al. in vivo assessment of hydroxyapatite and silicate-substituted hydroxyapatite granules using an ovine defect model. J Mater Sci Mater Med. 2005; 16 (5): 429-40.
Pezzatini S, Solito R, Morbidelli L, Lamponi S, Boanini E, Bigi A, et al. The effect of hydroxyapatite nanocrystals on microvascular endothelial cell viability and functions. J Biomed Mater Res A. 2006; 76 (3): 656-63.
Ratner BD. New ideas in biomaterials science - a path to engineered biomaterials. J Biomed Mat Res. 1993; 27 (7); 837-50.
Suchanek W, Yoshimura M. Processing and properties of hydroxyapatite-based biomaterials for use as hard tissue replacement implants. J Mater Res. 1998; 13 (1): 94-117.
Tadic D, Beckmann F, Schwarz K, Epple M. A novel method to produce hydroxyapatite objects with interconnecting porosity that avoids sintering. Biomaterials. 2004; 25 (16): 3335-40.
Wong CT, Chen QZ, Lu WW, Leong JC, Chan WK, Cheung KMC, et al. Ultrastructure study of mineralization of a strontium-containing hydroxyapatite (Sr-HA) cement in vivo . J Biomed Mater Res A. 2004; 70 (3): 428-35.
Wong CT, Lu WW, Chan WK, Cheung KMC, Luk KDK, Lu DS, et al. in vivo cancellous bone remodeling on a Strontium containing hydroxyapatite (Sr-HA) bioactive cement. J. Biomed Mater Res A. 2004; 68 (3): 513-21.
Xu JL, Khor KA, Sui JJ, Zhang JH, Chen WN. Protein expression profiles in osteoblasts in response to differentially shaped hydroxyapatite nanoparticles. Biomaterials. 2009; 30 (29): 5385-91.
Xue W, Moore JL, Hosick HL, Bose S, Bandyopadhyay A, Lu WW, et al. Osteoprecursor cell response to strontium-containing hydroxyapatite ceramics. J Biomed Mater Res A. 2006; 79 (4): 804-14.
Yamamoto MK, Luz JGC, Araujo VC. Bone tissue response to granular hydroxyapatite implanted in defects created in the mandible of rats. Rev Odontol Univ São Paulo. 1994; 8 (4): 281-6.
Zhang R, Zhou L, Li Q, Liu J, Yao W, Wan H. Upregulation of two actin- associated proteins prompts pulmonary artery smooth muscle cell migration under hypoxia. Am J Respir Cell Mol Biol. 2009; 41 (4): 467-75.
Este artigo é uma cópia com adaptação para o inglês do Artigo Original "MACHADO, Callinca Paolla Gomes et al. Avaliação da hidroxiapatita contendo estrôncio como substituto ósseo em tíbias de ovelhas. Innovations Implant Journal, v. 5, n. 1, p. 9-14, 2010." Esta Cópia com adaptação para o Português segue os preceitos Creative Commons CC BY 4.0 ( https://creativecommons.org/licenses/by/4.0/deed.pt_BR ) disponibilizado pelo periódico responsável pela publicação original ( http://revodonto.bvsalud.org/scielo.php?pid=S1984-59602010000100004&script=sci_arttext ).
INTEREST CONFLICTS
The authors declare no conflicts of interest
CONFLITOS DE INTERESSE
Os autores declaram não haver conflitos de interesse
This work is licensed under a Creative Commons Attribution 4.0 International License.
Copyright (c) 2019 Callinca Paolla Gomes Machado, Andrea Vaz Braga Pintor, Mônica Diuana Calasans Maia